AB0905 DEVELOPMENT OF A NEW COMPREHENSIVE ASSAY KIT FOR MYOSITIS-ASSOCIATED ANTIBODIES AND MYOSITIS-SPECIFIC ANTIBODIES EXAMINATION OF CLINICAL USEFULNESS IN INFLAMMATORY MUSCLE DISEASES

نویسندگان

چکیده

Background Inflammatory muscle diseases are systemic inflammatory disorders that affect not only muscles but also skin, joints, lungs, and heart. Dermatomyositis (DM) polymyositis (PM) characterized by the production of antibodies. Measurement these antibodies is useful for diagnosis, classification disease type, evaluation activity, prognosis prediction, determination therapeutic strategy. However, sensitivity specificity vary depending on measurement method, some autoantibodies can be measured in limited laboratories. Objectives We developed a novel comprehensive assay kit myositis-associated with aim developing rapid simple detection method myositis-specific or related autoantibodies. we used proteome-wide antibody screening quantification wet protein arrays consisting proteins synthesized from human cDNA library (HuPEX). examined whether this clinically compared to enzyme-linked immunosolbent (ELISA). Methods Sera 47 patients diagnosed suspected were measure (anti-ARS, anti-MDA5, anti-TIF1-γ, anti-Mi-2, anti-SRP, antibodies) myositis-related (anti-U1RNP, anti-Ku, anti-PM-Scl, anti-mitochondria using (HuPEX) results ELISA, which commercially available immunoprecipitation autoantibodies(anti-OJ, anti-Zo antibodies). Autoantibodies undetectable ELISA positive array subjected immunoprecipitation. In immunoprecipitation, primary added solution containing an antigen form antigen-antibody complex solution. Next, secondary immobilized beads adsorb beads. Finally, was eluted SDS analyzed SDS-PAGE. Results cases, clinical diagnosis DM 26 PM 20 inclusion body myositis 1 case. available, cases anti-ARS (17 DM, 3 PM), 5 anti-MDA5 (Amyopathic DM), case anti-TIF1-γ (DM), 2 Mi-2 (1 anti-RNP (PM), (PM). Compared eighteen system all other 100% concordant. addition, could detected anti-OJ system. Of 6 whose anti-HMGCR antibody-positive antibody-positive, consistent diagnosis. Furthermore, among 4 who anti-SS-A anti-SRP positive, anti-Ku anti-Ki Those samples confirmed Conclusion Collectively, vitro proteome reveal may provide biomarkers. References [1]Mimori T, idiopathic myopathy:an update pathophysiological significance. Curr Opin Rheumatol, 2007. 19: p. 523-529 [2]Fukuda E, et al. Identification characterization recognized germ cell mAb TRA98 array. Genes Cells, 2021. 26(3): p.180-189. Acknowledgements: NIL. Disclosure Interests None Declared.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Clinical Usefulness of Autoantibodies in Idiopathic Inflammatory Myositis

One of the most important characteristics of systemic autoimmune diseases, including idiopathic inflammatory myopathies (IIM), is the immune response to self-antigens manifested by the production of autoantibodies that recognize a variety of cytoplasmic and nuclear antigens. In the last 40 years, autoantibodies of patients with IIM have been investigated; however, some of the fundamental questi...

متن کامل

[Myositis specific and myositis associated autoantibodies in idiopathic inflammatory myopathies: a serologic study of 46 patients].

OBJECTIVE To characterize serum autoantibody profiles of patients with idiopathic inflammatory myopathies (IIM) by searching for myositis-specific (MSA) and myositis-associated (MAA) antibodies with sensitive and specific laboratory tests. METHODS We tested the sera from 46 Caucasian patients diagnosed as affected with IIM at the Division of Rheumatology of Padova University (21 polimyositis,...

متن کامل

significance of myositis - specific and myositis - associated autoantibodies

Autoantibodies to various cellular constituents are detected in the sera of patients with idiopathic inflammatory myopathy (IIM). These autoantibodies are closely associated with characteristic clinical manifestations of the diseases. Therefore, autoantibodies give us much information in clinical diagnosis, classification, prediction of prognosis and choice of treatment in patients with IIM. Du...

متن کامل

In adult onset myositis, the presence of interstitial lung disease and myositis specific/associated antibodies are governed by HLA class II haplotype, rather than by myositis subtype

The aim of this study was to investigate HLA class II associations in polymyositis (PM) and dermatomyositis (DM), and to determine how these associations influence clinical and serological differences. DNA samples were obtained from 225 UK Caucasian idiopathic inflammatory myopathy patients (PM = 117, DM = 108) and compared with 537 randomly selected UK Caucasian controls. All cases had also be...

متن کامل

Update on myositis-specific and myositis-associated autoantibodies.

Myositis-specific autoantibodies or myositis-associated autoantibodies can often be found in serum of patients with polymyositis and dermatomyositis. The presence of these autoantibodies can be significant in patient diagnosis and classification. Recent studies have provided new information about many of these specific autoantibodies. Among the more important developments were identification of...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Annals of the Rheumatic Diseases

سال: 2023

ISSN: ['1468-2060', '0003-4967']

DOI: https://doi.org/10.1136/annrheumdis-2023-eular.2028